The data is made up of 46595 cells consisting 3 Control samples
(22819 cells) and 4 Depleted samples (23776 cells)
36669 cells collected post processing meeting the requirements; - Less than < 15% mitochondrial gene content - Greater than > 500 genes and less than < 6000 genes expressed - Greater than > 1000 UMIs and less than < 25000s UMI expressed - Genes Expressed in a minimum of 10 cells (12978 genes)
The Data was transformed using 11 PC dimensions and 30 K-nearest
neighbors to generate a UMAP in 2 dimensions at a clustering resolution
of 0.6. The top 20 representing markers of each cluster were then
processed through CHATGpt for annotation.
Below are 2 explorable merged complete data object with dynamic legends. Double click on a cell type to isolate that cluster and single click to select/deselect a cluster. The colors of the complex object do not match the orginal object.
#Attempt at running Icytotrace on merged object
#Extracting barcode ids and plotting them back on seurat for scdata
#Cell Annotation